Kiwifruit resources and breeding innovation team from the Zhengzhou Fruit Research Institute published an article “Development of a 135K SNP genotyping array for Actinidia arguta and its applications for genetic mapping and QTL analysis in kiwifruit” in the Plant Biotechnology Journal.
Kiwifruit ( Actinidia spp) is a woody, perennial and dioecious vine. In this genus, there are multiple ploidy levels but the main cultivated cultivars are polyploid. Despite the availability of many genomic resources in kiwifruit, SNP genotyping is still a challenge given these different levels of polyploidy. In this study, we developed a high-density SNP genotyping array to facilitate genetic studies and breeding applications in kiwifruit. We screened a germplasm collection to identify SNPs across Actinidia species, filtered and selected 135K SNPs for the array. The array was used to genotype a tetraploid Actinidia arguta F1 population. An integrated linkage map was constructed covering 3060.9 cM across 29 linkage groups, showing a high degree of collinearity between the A. arguta genetic map and the A. chinensis Red5 reference genome. QTL mapping for the sex locus was performed that led to identification of a novel QTL on Linkage Group 3. Finally, the study presented evidence of tetrasomic inheritance with partial preferential pairing in A. arguta .
The genetic map of tetraploid Actindia arguta and comparison of the genetic map with the kiwifruit genome
The study was supported by the National Key Research and Development Project of China (2019YFD1000802); the National Natural Science Foundation of China (32001995) and the Science and Technology Innovation Project of CAAS (CAAS-ASTIP-2015-ZFRI).
By Wang Ran
wangran@caas.cn